کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4343486 | 1615107 | 2015 | 6 صفحه PDF | دانلود رایگان |

• DAMGO reduced GABAergic mIPSCs frequency in the VLO slices.
• DAMGO failed to alter GABAergic mIPSCs amplitude in the VLO slices.
• Pretreatment with CTOP reversed DAMGO induced effects in the VLO slices.
• Application of CTOP alone into the VLO slice did not produce any effect.
• Mu-opioid receptors would presynaptically inhibit the GABA release in the VLO.
Previous studies have indicated that mu-opioid receptors in the ventrolateral orbital cortex (VLO) are involved in antinociception in tail flick tests and GABAergic neurons or terminals express mu-opioid receptors in the VLO. The current study examined the effect of selective mu-opioid receptor agonist DAMGO on the GABAergic miniature inhibitory postsynaptic currents (mIPSCs) in the VLO in rats using the whole-cell patch clamp. The results demonstrated that 5 μM DAMGO application into the rat VLO slices significantly reduced the GABAergic mIPSCs frequency, without any effect on its amplitude, and this effect of DAMGO was reversed by pretreatment with selective mu-opioid receptor antagonist 1 μM CTOP. Importantly, application of CTOP alone into the VLO slices did not produce any effect on the frequency and amplitude of GABAergic mIPSCs. These results indicate a presynaptic effect of mu-opioid receptor activation on the GABAergic neurons in the VLO. The current data suggests that a presynaptic inhibition of the GABA release may contribute to the mu-opioid receptor mediated effects in the VLO and provides novel electrophysiological evidence for the underlying mechanisms of mu-opioid receptors in the VLO.
Journal: Neuroscience Letters - Volume 592, 10 April 2015, Pages 64–69