کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4344484 1296660 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Orexin-A excites pyramidal neurons in layer 2/3 of the rat prefrontal cortex
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Orexin-A excites pyramidal neurons in layer 2/3 of the rat prefrontal cortex
چکیده انگلیسی

The arousal peptides, orexins, play an important role in regulating the function of the prefrontal cortex (PFC). Although orexins have been shown to increase the excitability of deep-layer neurons in the medial prefrontal cortex (mPFC), little is known about their effect on layer 2/3, the main intracortical processing layer. In this study, we investigated the effect of orexin-A on pyramidal neurons in layer 2/3 of the mPFC using whole-cell recordings in rat brain slices. We observed that orexin-A reversibly depolarized layer 2/3 pyramidal neurons through a postsynaptic action. This depolarization was concentration-dependent and mediated via orexin receptor 1. In voltage-clamp recordings, the orexin-A-induced current was reduced by the replacement of internal K+ with Cs+, removal of external Na+, or an application of flufenamic acid (an inhibitor of nonselective cation channels). A blocker of Na+/Ca2+ exchangers (SN-6) did not influence the excitatory effect of orexin-A. Moreover, the current induced by orexin-A reversed near Ek when the external solution contained low levels of Na+. When recording with Cs+-containing pipettes in normal external solution, the reversal potential of the current was approximately −25 mV. These data suggest an involvement of both K+ channels and nonselective cation channels in the effect of orexin-A. The direct excitatory action of orexin-A on layer 2/3 mPFC neurons may contribute to the modulation of PFC activity, and play a role in cognitive arousal.


► Orexin-A directly excited the layer 2/3 pyramidal neurons in the rat mPFC.
► This excitatory effect was mediated via OX1R.
► The ionic mechanisms involved K+ channels and nonselective cation channels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 520, Issue 1, 27 June 2012, Pages 92–97
نویسندگان
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