کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4344508 1296662 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells
چکیده انگلیسی

DNA topoisomerase II alpha (Topo-IIα) is an essential nuclear enzyme with its up-regulation demonstrated in different tumors. This study is to evaluate the expression of Topo-IIα in glioblastoma cancer stem cells (CSCs) and its effects on cell proliferation and apoptosis. We observed the expression differences of Topo-IIα gene transcript in CSCs and non-CSCs in glioblastoma cell line U87 by quantitative real-time PCR and western blot; we silenced Topo-IIα expression in U87 CSCs using Topo-IIα-specific siRNA to investigate the role of Topo-IIα in cell proliferation, cell cycle, and apoptosis. We found higher expression of Topo-IIα in CSCs than in non-CSCs. After using Topo-IIα-specific siRNA to silence the expression of Topo-IIα gene, we observed decreasing cell proliferation, cell cycle arrest, and induction of apoptosis in CSCs, which suggest that Topo-IIα contributes to both cell proliferation and/or cell survival. Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas.


► We found higher expression of Topo-IIα in CSCs than in non-CSCs.
► Topo-IIα contributes to both cell proliferation and/or cell survival.
► Topo-IIα may be a novel marker of cell proliferation and/or survival factor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 518, Issue 2, 19 June 2012, Pages 138–143
نویسندگان
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