Neuroprotective effect of Jatrorrhizine on hydrogen peroxide-induced cell injury and its potential mechanisms in PC12 cells

The neuroprotective effects of Jatrorrhizine from Coptidis Rhizoma against hydrogen peroxide (H2O2)-induced rat pheochromocytoma line PC12 injury and its potential mechanisms were evaluated in the present study. When cells were exposed to H2O2 (200 μM) for 12 h, there was a significant reduction in cell survival and activity of antioxidant enzyme (SOD and HO-1) and LDH release. In addition, increased ROS production, declined MMP and increased production of malondialdehyde (MDA) were observed. Preincubation of cells with Jatrorrhizine (0.01–10.0 μM) 24 h prior to H2O2 exposure markedly elevated cell viability and activities of antioxidant enzyme (SOD and HO-1), prevented LDH release and lipid peroxidation (MDA) production, attenuated the decrease of MMP and scavenged ROS formation. Jatrorrhizine also attenuated caspase-3 activation of the downstream cascade following ROS. Our results suggest that Jatrorrhizine holds potential for neuroprotective effects against H2O2-induced injury.

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► Jatrorrhizine can scavenge DPPH radical in a concentration-dependent manner.
► Jatrorrhizine can inhibit the H2O2-induced intracellular accumulation of ROS in PC12 cells.
► Cleaved caspase-3 was decreased in PC12 cells which were pretreated with Jatrorrhizine.