کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4345546 1296739 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrophysiological analysis of vulnerability to experimental ischemia in neonatal rat spinal ventral horn neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Electrophysiological analysis of vulnerability to experimental ischemia in neonatal rat spinal ventral horn neurons
چکیده انگلیسی

To clarify the vulnerability of spinal motoneurons to excitotoxicity, we analyzed the agonal current induced by experimental ischemia in ventral lamina IX neurons of spinal cord slices from neonatal rats by using whole-cell patch-clamp. Ischemia was simulated in vitro by oxygen/glucose deprivation. Superfusion with ischemia-simulating medium elicited an agonal inward current, which was initially slow and then became rapid. We compared 8-, 9-, 10-, 11-, and 12-day postnatal rats and found age-dependent shortening of the latency of the rapid inward current. Furthermore, the membrane capacitance (Cm) and resting membrane potential (RMP) of the lamina IX neurons demonstrated significant negative correlations with the latency of the rapid inward current. Logistic regression analysis showed that postnatal age, Cm, and RMP were independent contributing factors to ischemic vulnerability. These results suggest that not only cell volume and ionic balance but also early postnatal maturation of the intracellular environment is vital for developing vulnerability to excitotoxicity.


► Experimental ischemia induces an agonal inward current in spinal lamina IX neurons.
► Cell volume is a contributing factor of vulnerability to ischemia.
► Resting membrane potential is a contributing factor of vulnerability to ischemia.
► There is a postnatal age-dependent development of vulnerability to ischemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 494, Issue 2, 25 April 2011, Pages 161–164
نویسندگان
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