کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4345569 | 1296740 | 2011 | 6 صفحه PDF | دانلود رایگان |

We have investigated the gene expression of the facilitated glucose transporter (GLUT), H+-coupled myo-inositol cotransporter (HMIT), and Na+ glucose cotransporter (SGLT) in the lateral wall of the cochlear duct by conventional RT-PCR and quantitative real-time PCR. The isoforms GLUT1, -3, -4, -5, -8, -10, -12 and HMIT were detected in both the stria vascularis and the spiral ligament, whereas no SGLT isoforms could be detected in these tissues. Quantitative real-time PCR analysis revealed significant differences in the gene expression of GLUT1, -4, -5, -10, and HMIT isoforms between the stria vascularis and the spiral ligament. This result reflects the tissue-dependent distributions of GLUT isoforms. These findings strongly suggest that a number of GLUT isoforms participate in glucose transport in the stria vascularis and the spiral ligament.
Research highlights▶ Multiple expression of GLUT isoform genes is detected in the cochlear lateral wall. ▶ Eight GLUT genes are expressed in both the stria vascularis and the spiral ligament. ▶ Quantitative analyses reveal the tissue-dependent distributions of the GLUT isoforms. ▶ A number of GLUT isoforms participate in glucose transport in the lateral wall.
Journal: Neuroscience Letters - Volume 490, Issue 1, 18 February 2011, Pages 72–77