کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4345770 | 1296753 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats
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کلمات کلیدی
nAChRMPGSodium/potassium ATPase[Ca2+]i - [Ca2 +] i[Na+]i - [Na +] IACh - آهAcetylcholine - استیل کولینafterhyperpolarization - بعد از پمپاژintracellular calcium ion concentration - غلظت یون کلسیم داخل سلولیmajor pelvic ganglion - لگن بزرگ لگنCa2+-activated K+ channel - کانال K + فعال شده Ca2 +Autonomic ganglia - گانگلیس خودمختاریnicotinic acetylcholine receptor - گیرنده استیلکولین نیکوتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The electrophysiological mechanism underlying afterhyperpolarization induced by the activation of the nicotinic acetylcholine receptor (nAChR) in male rat major pelvic ganglion neurons (MPG) was investigated using a gramicidin-perforated patch clamp and microscopic fluorescence measurement system. Acetylcholine (ACh) induced fast depolarization through the activation of nAChR, followed by a sustained hyperpolarization after the removal of ACh in a dose-dependent manner (10 μM to 1 mM). ACh increased both intracellular Ca2+ ([Ca2+]i) and Na+ concentrations ([Na+]i) in MPG neurons. The recovery of [Na+]i after the removal of ACh was markedly delayed by ouabain (100 μM), an inhibitor of Na+/K+ ATPase. Pretreatment with ouabain blocked ACh-induced hyperpolarization by 67.2 ± 5.4% (n = 7). ACh-induced hyperpolarization was partially attenuated by either the chelation of [Ca2+]i with BAPTA/AM (20 μM) or the blockade of small-conductance Ca2+-activated K+ channels by apamin (500 nM). Taken together, the activation of nAChR increases [Na+]i and [Ca2+]i, which activates Na+/K+ ATPase and Ca2+-activated K+ channels, respectively. Consequently, hyperpolarization occurs after the activation of nAChR in the autonomic pelvic ganglia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 482, Issue 2, 27 September 2010, Pages 167-171
Journal: Neuroscience Letters - Volume 482, Issue 2, 27 September 2010, Pages 167-171
نویسندگان
Kyu-Sang Park, Seung-Kuy Cha, Min-Jeong Kim, Na-Hyun Kim, Joong-Woo Lee, Seong-Woo Jeong, In Deok Kong,