کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4346699 1296800 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Opposite effects of nitric oxide on rod and cone photoreceptors of rat retina in situ
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Opposite effects of nitric oxide on rod and cone photoreceptors of rat retina in situ
چکیده انگلیسی
In the light-adapted vertebrate retina, nitric oxide (NO) modulates synaptic transmission between photoreceptors and second-order neurons. Although NO is believed to be a mediator of adaptation, its effect on photoreceptors in situ is not known yet. Therefore, we studied rod and cone activities in rat eyes in situ, using the electroretinogram (ERG). Rod and cone ERGs were functionally isolated by intravitreal 20 mM glutamate, which suppressed the activity of all retinal cells except rods and cones for about 90 min. The addition of NO-donor, SNAP, to the glutamate solution decreased the amplitude of the rod single-flash ERG by ∼40%, compared to the amplitude of the rod ERG isolated by glutamate alone, but it increased the amplitude of the isolated, intense paired-flash cone ERG by ∼40%. An excess of the NO-scavenger, CPTIO, had no significant effect on either rod or cone ERGs. A broad-spectrum NO-synthase inhibitor, L-NAME, increased the amplitude of the rod ERG by ∼50%, but had no significant effect on the cone ERG. We suggest that NO directly modulates the light-evoked activity of rod and cone photoreceptors in situ, but in opposite ways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 473, Issue 1, 31 March 2010, Pages 62-66
نویسندگان
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