کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4346713 1296801 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synaptic currents and transmitter responses in human NT2 neurons differentiated in aggregate culture
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Synaptic currents and transmitter responses in human NT2 neurons differentiated in aggregate culture
چکیده انگلیسی

Postmitotic neurons were generated from the human NT2 teratocarcinoma cell line in a novel cell aggregate differentiation procedure. The NT2 model neurons express punctate immunoreactivity for synapsin and for cell markers related to GABAergic and glutamatergic neurotransmission. Using the outside-out patch-clamp configuration, we characterized the kinetics of currents elicited by a rapid application of the amino acid neurotransmitters. Moreover, we detected spontaneous postsynaptic currents in glia free cell cultures that may result from the firing activity of glutamatergic and GABAergic NT2 neurons. These cultured spontaneously active networks may be a useful tool to analyze factors that modulate the formation and efficacy of synapses between human neurons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 468, Issue 3, 14 January 2010, Pages 207–210
نویسندگان
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