کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4347477 1296843 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of retinal cells from mouse and human induced pluripotent stem cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Generation of retinal cells from mouse and human induced pluripotent stem cells
چکیده انگلیسی

We previously reported a technique for generating retinal pigment epithelia (RPE) and putative photoreceptors from embryonic stem (ES) cells. Here we tested whether our procedure can promote retinal differentiation of mouse and human induced pluripotent stem cells (iPSCs). Treating iPSCs with Wnt and Nodal antagonists in suspension culture induced expression of markers of retinal progenitor cells and generated RPE cells. Subsequently, treatment with retinoic acid and taurine generated cells positive for photoreceptor markers in all but one human cell lines. We propose that iPSCs can be induced to differentiate into retinal cells which have a possibility to be used as patient-specific donor cells for transplantation therapies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 458, Issue 3, 24 July 2009, Pages 126–131
نویسندگان
, , , , , , , ,