کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4347622 1296852 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Orexins increase mRNA expressions of neurotrophin-3 in rat primary cortical neuron cultures
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Orexins increase mRNA expressions of neurotrophin-3 in rat primary cortical neuron cultures
چکیده انگلیسی

Orexins and melanin-concentrating hormone (MCH) as orexigenic neuropeptides are present in the lateral hypothalamus, and their receptors are distributed in the cerebral cortex and hippocampus. In the present study, the regulatory effects of orexin-A, orexin-B and MCH on neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) expressions were examined in primary cortical neuron cultures using quantitative real-time PCR. Both orexin-A and orexin-B on 6-day exposure significantly increased the NT-3 mRNA at concentrations of 0.01, 0.1 and 1 μM. Orexin-A and B at 1 μM led to an increase of twofold or more over the control. However, no such NT-s mRNA increase occurred with exposure to MCH at the same concentrations as orexins. The mRNA expression of BDNF was significantly increased only by orexin-B at 1 μM. These findings suggest that orexins, but not MCH, may be an inducer of NT-3 in the cerebral cortex.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 450, Issue 2, 30 January 2009, Pages 132–135
نویسندگان
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