کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4348322 | 1296885 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effects of ouabain on resting membrane potential and hyperpolarization-activated current in neonatal rat nodose ganglion neurons
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
To determine whether the responses of resting membrane potential (RMP) and hyperpolarization-activated current (IH) are altered by the application of ouabain, one of the Na+-K+ pump inhibitors, in neonatal rat small-diameter (<30 μm) nodose ganglion (NG) neurons, we examined the effects of 1 μM ouabain on those responses using perforated patch-clamp techniques. In current-clamp mode, the RMP was 40.2 ± 1.6 mV (n = 31). Twenty of 31 cells tested were depolarized by ouabain application, and these responses were associated with an increase in the cell input resistance. In the remaining 11 cells studied, 3 showed hyperpolarization in response to ouabain and 8 showed no effect on RMP. In voltage-clamp mode, 1 μM ouabain application enhanced the IH in all of 10 neurons examined. These results suggest that ouabain application at 1 μM is capable of setting both the RMP level and the neuronal excitability in small-diameter NG neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 439, Issue 3, 18 July 2008, Pages 241-244
Journal: Neuroscience Letters - Volume 439, Issue 3, 18 July 2008, Pages 241-244
نویسندگان
Shigeji Matsumoto, Junichi Kitagawa, Mamoru Takeda,