کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4351103 | 1297007 | 2006 | 5 صفحه PDF | دانلود رایگان |
Whole-cell patch-clamp and local electrical stimulation were used for measuring of monosynaptic GABAergic currents from rat hippocampal neurons in culture. Under control conditions (normal extracellular calcium, 2 mM) paired-pulse depression with 150 ms interpulse interval was observed. The mean current amplitude for both 1st and 2nd IPSCs displayed bell-shaped dependency from the stimulus amplitude. When extracellular Ca was either decreased to 0.5 mM or increased to 5 mM both mean amplitude of IPSCs and mean release probability decreased/increased correspondingly yet their dependences from the stimulus strength remained bell-shaped. Mean paired-pulse ratio was also affected—we observed facilitation of second IPSC in low-calcium whereas the latter was depressed in high-calcium solution. Our results suggest that calcium concentrations not only regulate the strength of paired-pulse plasticity but also can invert its direction.
Journal: Neuroscience Letters - Volume 395, Issue 2, 6 March 2006, Pages 133–137