کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4351586 1615307 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Superoxide generation in different brain regions of rats during normoxia and hypoxia-reoxygenation
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Superoxide generation in different brain regions of rats during normoxia and hypoxia-reoxygenation
چکیده انگلیسی

The superoxide-dependent chemiluminescent intensity in different brain regions was examined in ex vivo tissue slices of rat brain during normoxia and hypoxia-reoxygenation with lucigenin. The chemiluminescent intensity increased during reoxygenation after hypoxic treatment. There was a higher level of chemiluminescent intensity in the hippocampus during normoxia, and a lower level in the white matter during normoxia and hypoxia-reoxygenation. A weak correlation was found between the chemiluminescent intensity and the glucose uptake rate during normoxia. Then we examined whether hypoxic strength correlates to superoxide generation. The chemiluminescent intensity increased in a hypoxic strength-dependent manner. The generation mechanism of superoxide was examined using carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial uncoupler, genipin, an inhibitor for uncoupling protein-2, alloprinol, a xanthine oxidase inhibitor, or apocynin, an NADPH oxidase inhibitor. The chemiluminescent signal was significantly inhibited by CCCP under normoxic condition and enhanced by genipin during normoxia and hypoxia-reoxygenation, but not by allopurinol or apocynin.These results suggest that superoxide generation is high in the hippocampus during normoxia and low in the white matter during normoxia and hypoxia-reoxygenation, superoxide generation in the hypoxia-reoxygenation brain correlates with the strength of hypoxia influenced by oxygen delivery, and mitochondrion is the major sites of intracellular superoxide generation.


► Superoxide generation is high in hippocampus and low in white matter.
► Superoxide generation during reoxygenation increased in a hypoxic strength-dependent manner.
► Mitochondrion is one of the major sites of intracellular superoxide generation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Research - Volume 74, Issues 3–4, December 2012, Pages 261–268
نویسندگان
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