Ensemble neural activity of the frontal cortical basal ganglia system predicts reaction time task performance in rats

The question pursued in this study was when neural activity appears in the cortico-basal ganglia system that could predict alternate behavioral responses in a reaction time (RT) task. In this protocol, rats first performed a nose poke to initiate a trial, depressed a lever when presented, and then released the lever after a tone cue. Multiple-channel, single-unit recordings (up to 62 units) were obtained simultaneously from the prefrontal cortex, the dorsal medial striatum, the globus pallidus, and the substantia nigra pars reticulata in a single rat during a session. Results indicated that (1) global alterations of neural activity appeared in clusters, which was associated with different behavioral components and observed in each of the targeted areas; (2) small independent subsets of neurons responded differently between error (lever was released before tone presentation) and correct trials (lever was released within 0.5 s after tone onset) during these behavioral episodes; (3) significant correlations between RTs and single units activities were found in the early preparation phases of the task. The results reveal that complex early preparatory activity exists several seconds before the final movements in a RT task, which may determine executive functions leading to rapid decoding of alternate behavioral performances.

► Rats were trained to perform a reaction time task, releasing a lever after tone cue.
► Multiple-channel, single unit recordings were obtained from mPFC, DMS, GP and SNr.
► Neural responses associated with behavioral components exhibited in clusters.
► Small subsets of neurons responded differently between error and correct trials.
► Correlations between reaction times and neural activities were found in early phase.