Sound transmission along the ossicular chain in common wild-type laboratory mice

The use of genetically modified mice can accelerate progress in auditory research. However, the fundamental profile of mouse hearing has not been thoroughly documented. In the current study, we explored mouse middle ear transmission by measuring sound-evoked vibrations at several key points along the ossicular chain using a laser-Doppler vibrometer. Observations were made through an opening in pars flaccida. Simultaneously, the pressure at the tympanic membrane close to the umbo was monitored using a micro-pressure-sensor. Measurements were performed in C57BL mice, which are widely used in hearing research. Our results show that the ossicular local transfer function, defined as the ratio of velocity to the pressure at the tympanic membrane, was like a high-pass filter, almost flat at frequencies above ∼15 kHz, decreasing rapidly at lower frequencies. There was little phase accumulation along the ossicles. Our results suggested that the mouse ossicles moved almost as a rigid body. Based on these 1-dimensional measurements, the malleus–incus-complex primarily rotated around the anatomical axis passing through the gonial termination of the anterior malleus and the short process of the incus, but secondary motions were also present.This article is part of a Special Issue entitled “MEMRO 2012”.

► Local transfer function (LTF) was mapped along the ossiclar chain in wild type mice.
► The pressure at the tympanic membrane was quantified using a micro-pressure sensor.
► Simultaneously, the ossicular velocity was measured by Laser-Doppler vibrometer.
► The LTF was smooth and like a high-pass filter.
► There was little phase accumulation along the ossicles.