کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4355965 | 1615652 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aging outer hair cells (OHCs) in the Fischer 344 rat cochlea: Function and morphology
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کلمات کلیدی
PBSARHLOHCDPOAESDHF344SGCmETCPbSbI/O - I / OROS - ROSPrestin - آماده استAge-related hearing loss - افت شنوایی مرتبط با سنdistortion product otoacoustic emission - انتشار محصولات octo آکوستیک محصول اعوجاجIHC - ایمونوهیستوشیمیAlternative Current - جریان متناوبstandard error of the mean - خطای استاندارد میانگینmitochondrial electron transport chain - زنجیره حمل و نقل الکترون الکترونیک میتوکندریSOD - سدouter hair cell - سلول بیرونی موInner hair cell - سلول داخلی داخلیSpiral ganglion cell - سلول گانگلیونی اسپیرالsuperoxide dismutases - سوکسوکس دیسموتازهاsuccinate dehydrogenase - سوکسیناد دهیدروژنازphosphate buffer saline - فسفات بافر شورFischer 344 - فیشر 344Fischer 344 rat - فیشر 344 موشSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیCochlear microphonics - میکروفونیک Cochlearinput/output - ورودی خروجیendocochlear potential - پتانسیل آندوکولارCompound Action Potential - پتانسیل اقدام مشترکCaP - کلاه لبه دارReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
سیستم های حسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
As previously reported [Popelar, J., Groh, D., Pelanova, J., Canlon, B., Syka, J., 2006. Age-related changes in cochlear and brainstem auditory functions in Fischer 344 rats. Neurobiol. Aging 27, 490-500; Buckiova, D., Popelar, J., Syka, J., 2007. Aging cochleas in the F344 rat: morphological and functional changes. Exp. Gerontol. 42, 629-638; Bielefeld, E.C., Coling, D., Chen, G.D., Li, M.N., Tanaka, C., Hu, B.H., Henderson, D., 2008. Age-related hearing loss in the Fischer 344/NHsd rat substrain. Hear. Res. 241, 26-33], aged Fischer 344 (F344) rats with severe hearing loss retain many outer hair cells (OHCs) especially in the middle turn of the cochlea. The current study confirmed the previous findings showing that aged OHCs were present, but dysfunctional. Distortion product otoacoustic emissions (DPOAE), which are believed to reflect in vivo OHC motility, were absent in the aged rats while the majority of OHCs (>80%) were present and morphologically intact. There was no detectable injury of OHC stereocilia as assessed by actin-staining and examination under the light microscope. Cochlear microphonics (CM) at 12Â kHz, recorded from the middle turn, only showed a slight age-related reduction, indicating a normal mechanoelectrical transduction apparatus in the remaining OHCs in the cochlear regions with 10-20% OHC loss. Activities of succinate dehydrogenase (SDH), an enzyme shared by the citric acid cycle and the mitochondrial electron transport chain (METC), were also at normal levels in aged OHCs. Importantly, aged OHCs showed reduced levels of prestin immunolabeling compared to young controls. Together with our previous finding showing that the stria vascularis and endocochlear potential were essentially normal in aged F344 rats [Bielefeld, E.C., Coling, D., Chen, G.D., Li, M.N., Tanaka, C., Hu, B.H., Henderson, D., 2008. Age-related hearing loss in the Fischer 344/NHsd rat substrain. Hear. Res. 241, 26-33], the results suggest that disruption of prestin is the major cause of DPOAE loss and loss of cochlear sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 248, Issues 1â2, February 2009, Pages 39-47
Journal: Hearing Research - Volume 248, Issues 1â2, February 2009, Pages 39-47
نویسندگان
Guang-Di Chen, Manna Li, Chiemi Tanaka, Eric C. Bielefeld, Bo-Hua Hu, Mohammad Habiby Kermany, Richard Salvi, Donald Henderson,