کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4357149 | 1300036 | 2011 | 7 صفحه PDF | دانلود رایگان |
Several Ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. A lectin from Penicillium chrysogenum, named PeCL, was purified from a fungal culture using gel-filtration chromatography column. PeCL was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visualised on SDS-PAGE gel. Purified PeCL fraction was delivered via artificial diet to Myzus persicae aphid and was demonstrated to be aphicidal at 0.1 % with higher toxic efficiency than a known mannose-binding lectin Concanavalin A (ConA). A fast and efficient way to purify PeCL and a potential use in pest control is described.
► Focus on Ascomycetes fungi used in bio-industries.
► Use of available industrial residues for lectin extraction.
► Penicillium chrysogenum lectin PeCL simply and efficiently purified.
► Mannose specific binding property determined for PeCL.
► Aphicidal effect for PeCL for pest biocontrol.
Journal: Fungal Biology - Volume 115, Issue 11, November 2011, Pages 1093–1099