کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357298 1300049 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structures of wall heterogalactomannans isolated from three genera of entomopathogenic fungi
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Structures of wall heterogalactomannans isolated from three genera of entomopathogenic fungi
چکیده انگلیسی

O-linked heterogalactomannans with similar structural features have been purified from the fungal walls of the entomopathogenic fungi Lecanicillium muscarium, Beauveria bassiana, Beauveriabrongniartii, and Cordyceps sphingum. Their composition and structure have been determined using acid hydrolysis, methylation analysis, gas-liquid chromatography-mass spectrometry (GC-MS) and Nuclear magnetic resonance spectroscopy (NMR). All structures have an α-(1 → 6)-mannose backbone, but one of the two strains of L. muscarium included in this study contained an acidic heterogalactomannan instead of the neutral polysaccharide isolated in the rest of the species analyzed. Sequence analysis of the internal transcribed spacer (ITS) region of this strain indicated that it belongs to the related genus Simplicillium, displaying low identity (83 %) with the closest Lecanicillium species. This is a new demonstration of the structural diversity of fungal wall heteromannans and validates their interest as chemotaxonomic markers. The production of a pullulan-like extracellular polysaccharide in strain CBS 413.70C of L. muscarium is also reported.


► O-linked polysaccharides from several entomopathogenic fungi have been analyzed.
► These heteromannans had similar structures, except in one strain of L. muscarium.
► This strain was identified through ITS sequencing as Simplicillium lanosoniveum.
► It can be concluded that cell wall heteromannans can provide valuable chemotaxonomical information.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Biology - Volume 115, Issue 9, September 2011, Pages 862–870
نویسندگان
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