کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357313 1300051 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The genetic profile and molecular diagnosis of thiophanate-methyl resistant strains of Fusarium asiaticum in Japan
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
The genetic profile and molecular diagnosis of thiophanate-methyl resistant strains of Fusarium asiaticum in Japan
چکیده انگلیسی

Fusarium asiaticum strains resistant to thiophanate-methyl were detected in four prefectures in Japan though their proportion in the total population was low in all instances. The F167Y or F200Y mutation in the β2-tubulin gene (FGSG06611.3) was detected in thiophanate-methyl resistant (TMR) strains. A PCR-based diagnostic method based on these mutations was developed and applied for all 17 TMR strains that have been detected so far in Japan. Three and 14 TMR strains were the F167Y and F200Y mutation types, respectively. Analysis by 11 variable number of tandem repeat markers showed that TMR strains from the same site had an identical genotype, while TMR strains from different sites were dissimilar. This result indicates that TMR strains did not spread clonally to the different sites.


► The F167Y or F200Y mutation in the β2-tubulin gene was detected in thiophanate-methyl resistant (TMR) Fusarium asiaticum.
► Three and 14 TMR strains were the F167Y and F200Y mutation types, respectively.
► TMR strains from the same site had an identical genotype, while TMR strains from different sites were dissimilar.
► Result indicates that TMR strains did not spread clonally to the different sites.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Biology - Volume 115, Issue 12, December 2011, Pages 1244–1250
نویسندگان
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