کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357382 1300055 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CDR4 is the major contributor to azole resistance among four Pdr5p-like ABC transporters in Neurospora crassa
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
CDR4 is the major contributor to azole resistance among four Pdr5p-like ABC transporters in Neurospora crassa
چکیده انگلیسی

Pdr5p-like ABC transporters play a significant role in azole resistance in Saccharomyces cerevisiae and Candida spp. Most of filamentous fungal species have multiple Pdr5p homologues. In this study, phylogenic analysis identified that filamentous fungi have at least two phylogenically distant groups of Pdr5p homologues. One contains PMR1-like Pdr5p homologues while the other contains both AtrF-like and AtrB-like Pdr5p homologues. Neurospora crassa has a total of four genes encoding Pdr5p homologues including CDR4 (PMR1-like), ATRB (AtrB-like), and ATRF (AtrF-like) and ATRF-2 (AtrF-like). By analyzing the susceptibilities of their knockout mutants to azole drugs including ketoconazole, fluconazole, and itraconazole, we found that deletion of cdr4 increased the susceptibility to antifungal azoles. In contrast, neither single-gene nor triple-gene deletion of atrb, atrf, and atrf-2 could not alter the susceptibility to azoles. In addition, cdr4, but not other Pdr5p homologue-coding genes, responded transcriptionally to ketoconazole stress. Together with the previous findings in other fungal species, these results suggest that the PMR1-like but not the AtrF-like or AtrB-like Pdr5p homologues play a key role in antifungal azole resistance in filamentous fungi.


► Filamentous fungi have two phylogenically distant groups of Pdr5p homologues.
► Neurospora crassa has four Pdr5p homologues.
► CDR4 is the only Pdr5p homologue mediating azole resistance.
► cdr4 is the only Pdr5p homologue-coding gene transcriptionally responsive to ketoconazole stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Biology - Volume 116, Issue 7, July 2012, Pages 848–854
نویسندگان
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