کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357397 1300056 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Grafting as a method for studying development in the filamentous fungus Podospora anserina
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Grafting as a method for studying development in the filamentous fungus Podospora anserina
چکیده انگلیسی

While grafting and transplant experiments have extensively been used to study development in animals and plants, they have seldom been employed to study fungal development. Here, grafting is used to study the interplay between mycelium and multicellular fruiting bodies during maturation in the model ascomycete Podospora anserina. Data indicate that grafts need a competent mycelium to continue their ripening. Vegetative incompatibility does not prevent transplanted fructifications to undergo development. Grafting onto mutant mycelia confirmed a previous model stating that the NADPH oxidase PaNox1 is required in the developing fruiting bodies, while the MAP kinase cascade PaMpk1 is required in the mycelium. Data also show that the IDC1 protein is required not only in the developing fruiting bodies but also in the mycelium, likely because of its role in anastomosis. Finally, entry inside the grafted fruiting bodies of a ribosomal protein tagged with GFP could be detected, suggesting that cellular components are imported from the underlying mycelium during maturation.


► A novel method for grafting fruiting bodies onto non-parental mycelium is presented.
► The method allows dissecting nutrient requirement for fructification development.
► It permits to test the mode of action of genes involved in fruiting body formation.
► It enable to follow traffic in and out maturing fruiting bodies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Biology - Volume 115, Issue 8, August 2011, Pages 793–802
نویسندگان
,