کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357572 1300093 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an activation tagging system for the basidiomycetous medicinal fungus Antrodia cinnamomea
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Development of an activation tagging system for the basidiomycetous medicinal fungus Antrodia cinnamomea
چکیده انگلیسی
This study describes the development of an efficient and reliable activation tagging system for the medicinal fungus Antrodia cinnamomea. For successful Agrobacterium tumefaciens-mediated transformation, different parameters were considered. The Agrobacterium concentration of 5 × 108 cfu ml−1, 1 mm acetosyringone, 25-d-old mycelia at 0.2 g ml−1, and co-culture period of 6 d were found to be the most optimal conditions for enhancing the transformation efficiency. The mitotic stability of transferred DNA (T-DNA) was demonstrated by growing eight randomly selected putative transformants in malt extract agar medium for five subcultures. Insertion of T-DNA into the genome of transformants was confirmed by PCR and Southern hybridization. Results showed that 88 % of the mutants contained a single T-DNA insertion. Two of the mutants were observed with different triterpenoid profiles compared with the untransformed cultures. Our results suggest a new functional genomics approach to tag the triterpenoid biosynthesis genes in A. cinnamomea.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycological Research - Volume 113, Issue 3, March 2009, Pages 290-297
نویسندگان
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