کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357783 1300105 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multiple isoforms of malic enzyme in the oleaginous fungus, Mortierella alpina
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Multiple isoforms of malic enzyme in the oleaginous fungus, Mortierella alpina
چکیده انگلیسی

Malic enzyme (ME; E.C. 1.1.1.40) is the only enzyme that can provide NADPH for fatty acid biosynthesis in oleaginous micro-organisms. However, it can simultaneously fulfil other roles and may thus exist in different forms, possibly coded for by different genes. At least seven isoforms (A–G) of ME were identified in the oleaginous fungus, Mortierella alpina, using a specific activity stain following non-denaturing polyacrylamide gel electrophoresis (PAGE) of extracts of cells grown under different conditions. Only isoform E, which arises from isoform D, was associated with lipid accumulation, becoming evident after nitrogen depletion from the medium and, under which conditions, lipid accumulation occurs. Isoforms A, B, C, F, and G were associated with oxygen-limited growth. Isoforms D and E occurred under both anaerobic and aerobic growth conditions. During the storage of the whole cells at −20 °C, isoform E was gradually converted to isoform G suggesting that a further post-transcriptional modification of the protein was occurring.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycological Research - Volume 112, Issue 6, June 2008, Pages 725–730
نویسندگان
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