کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4364294 1616311 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The function of an installed photosynthetic formaldehyde-assimilation pathway containing dihydroxyacetone synthase and dihydroxyacetone kinase enhanced formaldehyde removal from solution in transgenic geranium leaves
ترجمه فارسی عنوان
عملکرد یک فرآیند جذب فرمالدئید فتوسنتزی نصب شده حاوی دی هیدروکسی سکتون سینتاز و دی هیدروکسی استون کیناز افزایش فرمالدئید را از محلول در برگ های تراریخته ژرانیم
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
چکیده انگلیسی


• HCHO-uptake kinetics by DAS/DAK leaves from a HCHO solution was investigated.
• HCHO can be assimilated via the DAS/DAK pathway under a short stress of liquid HCHO.
• Original HCHO metabolic pathways did not function in liquid HCHO-treated transgenic leaves.
• Function of the DAS/DAK pathway enhanced HCHO uptake by transgenic leaves.
• Contribution of the DAS/DAK pathway to HCHO uptake by DAS/DAK leaves was evaluated.

In a previous study, we created transgenic geranium leaves installed with a photosynthetic formaldehyde (HCHO)-assimilation pathway containing dihydroxyacetone synthase (DAS) and dihydroxyacetone kinase (DAK). In the current study, DAS and DAK expression was confirmed in the leaves of three selected transgenic lines via RT-PCR analysis. 13C NMR analysis showed that the actions of the DAS/DAK pathway completely inhibited the role of citrate-produced pathway during HCHO metabolism in transgenic geranium leaves treated with 2 mM H13CHO for 4 h. Further analysis indicated that HCHO uptake from this solution was always higher in the leaves of the three transgenic lines than in WT (untransformed) leaves during a 48-h treatment period. This result suggests that the role of the installed DAS/DAK pathway enhanced HCHO uptake from solution in transgenic geranium leaves. Moreover, the relationship between HCHO uptake and treatment time approached a linear function during the first 40-h treatment period due to the function of the installed pathway. Based on the H13CHO metabolic profile and the HCHO uptake curves, H13CHO metabolic pathways in transgenic leaves treated with 2 mM H13CHO were postulated and the contribution of the DAS/DAK pathway to HCHO uptake by transgenic leaves was quantitatively evaluated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 106, January 2016, Pages 127–132
نویسندگان
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