Vegetation restoration and prevention of coastal sand dunes erosion using ion exchange resins and the plant growth-promoting rhizobacteria Bacillus sp. SH1RP8 isolated from indigenous plants

• Bacillus tequilensis SH1RP8, which have strong salt tolerance, and evaluated the plant growth-promoting ability.
• SH1RP8 enhanced the growth of P. japonicum planted in general soil, up 10.9%, compared to control.
• SH1RP8 increased the stem dry weights of P. japonicum planted in sand dune soil by 25%, compared to control.
• For Arundo donax, length increased by 6.4%, dry weight of stems by 20%, and roots dry weight by 30.8%, compared to control.
• For P. japonicum, length increased by 89.2%, and weights of stems by 91.5%, compared to control.

In this study, Bacillus sp. SH1RP8, which showed salt tolerance toward 8% of salinity, was isolated and evaluated the plant growth-promoting ability, IAA producibility, active ACC deaminase and siderophores synthesis. Bacillus sp. SH1RP8 enhanced the shoot growth and dry weight of Peucedanum japonicum, the representative coastal sand dune plant, planted in general soil, up 10.9% and 51.7%, compared to control, inoculated without rhizobacteria. Furthermore, SH1RP8 increased the shoot dry weights of P. japonicum planted in sand dune soil up 25%, compared to control. For Arundo donax, SH1RP8 increased the shoot dry weight up 20%, and roots dry weight up 30.8%, compared to control, which is significant.Also, the enhancement rates of the shoot length of P. japonicum were calculated as 73.6%, 73.2%; and dry weights as 89.2% and 91.5% in the control 2 (treated with ion exchange resins) and in the experimental group (treated with resins and rhizobacteria), respectively.By the analysis of DGGE, in the soil where P. japonicum was cultivated with the inoculation of SH1RP8, especially, while the soil where only P. japonicum was raised showed nothing, new bands were appeared. As a result of identification of these bands, they showed the most similarity with Bacillus sp.