کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4364645 | 1616318 | 2015 | 12 صفحه PDF | دانلود رایگان |

• The ability of Bacillus pumilus ACA-DC 4061 to promote biomineralization of calcium carbonate was investigated.
• Constant formation of vaterite was identified that remained stable in samples even after a year.
• Design of experiments was applied engaging three main factors: temperature, medium and inoculum concentration.
• Optimization of obtained statistical model showed that vaterite provided encouraging bioconsolidation results on marble.
In this work, Bacillus pumilus ACA-DC 4061 was selected for its increased capability for biomineralization on marble, under different nutrient media concentrations and temperature conditions. The optimum conditions for the CaCO3 bacterially-induced precipitation were determined with the aid of testing based on the Design of Experiments (DoE). Biomineral (vaterite) precipitation was favored in both the temperatures (25 and 30 °C) investigated. Stone loss rate was reduced when the samples were subjected to sonication. Thin sections of the substrate confirmed that vaterite was able to adhere onto the surface. Finally, under non-sterile conditions mimicking an in situ application, B. pumilus ACA-DC 4061 formed a fine layer of calcium carbonate. Therefore, this microorganism showed that vaterite formation may consistently occur under specific conditions and could prove useful as a candidate for on-site applications for stone conservation.
Journal: International Biodeterioration & Biodegradation - Volume 99, April 2015, Pages 73–84