کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4364645 1616318 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vaterite bio-precipitation induced by Bacillus pumilus isolated from a solutional cave in Paiania, Athens, Greece
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Vaterite bio-precipitation induced by Bacillus pumilus isolated from a solutional cave in Paiania, Athens, Greece
چکیده انگلیسی


• The ability of Bacillus pumilus ACA-DC 4061 to promote biomineralization of calcium carbonate was investigated.
• Constant formation of vaterite was identified that remained stable in samples even after a year.
• Design of experiments was applied engaging three main factors: temperature, medium and inoculum concentration.
• Optimization of obtained statistical model showed that vaterite provided encouraging bioconsolidation results on marble.

In this work, Bacillus pumilus ACA-DC 4061 was selected for its increased capability for biomineralization on marble, under different nutrient media concentrations and temperature conditions. The optimum conditions for the CaCO3 bacterially-induced precipitation were determined with the aid of testing based on the Design of Experiments (DoE). Biomineral (vaterite) precipitation was favored in both the temperatures (25 and 30 °C) investigated. Stone loss rate was reduced when the samples were subjected to sonication. Thin sections of the substrate confirmed that vaterite was able to adhere onto the surface. Finally, under non-sterile conditions mimicking an in situ application, B. pumilus ACA-DC 4061 formed a fine layer of calcium carbonate. Therefore, this microorganism showed that vaterite formation may consistently occur under specific conditions and could prove useful as a candidate for on-site applications for stone conservation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 99, April 2015, Pages 73–84
نویسندگان
, , , , , , , ,