کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4364803 1301725 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Degradation and detoxification of aflatoxin B1 by Pseudomonas putida
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Degradation and detoxification of aflatoxin B1 by Pseudomonas putida
چکیده انگلیسی


• Pseudomonas putida can tolerate up to 0.2 μg ml−1 concentration of AFB1 in the culture medium.
• P. putida transformed AFB1 into much less toxic compound.
• The AFB1 gave 90% cytotoxicity, where as the equivalent amount of biotransformed compound did not show any cytotoxicity.
• The bio-transformed AFB1 was identified using TLC, UV-Spectrometry, HPLC, FT-IR and GC–MS as AFD1, AFD2 and AFD3.

Aflatoxin is a mycotoxin produced by Aspergillus flavus and a common contaminant of food and feed, posing health hazards to humans and animals alike. The aim of this study is to explore the ability of Pseudomonas putida to degrade aflatoxin B1 (AFB1). The toxigenic strain of A. flavus was isolated from sugarcane and used to produce AFB1 in yeast extract sucrose medium. Two P. putida strains, MTCC 1274 and 2445, were cultured in mineral salt glucose medium (MSG) containing AFB1. The AFB1 was analyzed qualitatively and quantitatively by TLC and HPLC. It was found that Pseudomonas sp. can tolerate AFB1 in the medium (0.2 μg ml−1), and degrades it very efficiently. Within 24 h of incubation, AFB1 was reduced to an undetectable level in MSG medium. Analysis with TLC, HPLC, UV spectrophotometer, gas chromatography mass spectrometry (GC–MS), and Fourier transform infra-red spectroscopy (FT-IR) showed that AFB1 was bio-transformed to structurally different compounds (AFD1, AFD2, and AFD3), with the modified furan and lactone ring on the AFB1 molecule. A toxicity study on the HeLa cells showed that the new compounds formed are less toxic when compared with AFB1.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 86, Part C, January 2014, Pages 202–209
نویسندگان
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