کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4364923 1616328 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Shift in microbial population in response to crystalline cellulose degradation during enrichment with a semi-desert soil
ترجمه فارسی عنوان
تغییر در جمعیت میکروبی در پاسخ به تخریب بلورین سلولز در طول غنی سازی با یک خاک نیمه بیضی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
چکیده انگلیسی


• Bacterial community developed from semi-desert soil showed degradation of crystalline cellulose.
• Addition of crystalline cellulose induced a shift in the glycosyl hydrolase population.
• Semi-qPCR showed significant increase in both glycosyl hydrolase and 16S rRNA gene copy number during cellulose degradation.

Addition of crystalline cellulose to semi-desert soil shifts the microbial population; this was assessed by following the 16S rRNA gene, glycosyl hydrolase, and measuring its functional diversity in the bacterial population. Quantification of the glycosyl hydrolase gene showed an increase from 1 × 104 g−1 of unamended soil to 3 × 104 g−1 of crystalline-cellulose-amended soil by the 15th day of crystalline cellulose utilization. The indigenous glycosyl hydrolase community in unamended soil was dominated by the clone families that were closely related to the glycosyl hydrolases from Betaproteobacteria and Firmicutes. The addition of crystalline cellulose induced a shift in the glycosyl hydrolase population toward an increase in the relative abundance of the glycosyl hydrolase that was consistent with those of Bacteroidetes and Flavobacteria. The population shift of glycosyl hydrolase was also supported by the comparison of the 16S rRNA gene families in unamended and crystalline-cellulose-amended soil libraries. The most abundant 16S rRNA gene sequences retrieved in the unamended soil were identical to Pseudomonas, Massilia, Paenibacillus, and Bacillus spp., while Cytophaga and Flavobacterium spp. dominated in crystalline-cellulose-amended soil.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 88, March 2014, Pages 134–141
نویسندگان
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