Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp. D4 and cloning of its cnb genes

► The 4-CNB-degrader strain D4 was first reported from the genus of Cupriavidus sp. ► Cupriavidus sp. D4 could degrade 4-CNB in presence of heavy metals. ► The main 4-CNB degrading related genes of strain D4 were cloned and analyzed. ► The main 4-CNB degrading related genes were located on two plasmids.