کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4365398 | 1301758 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation of a biphenyl-degrading bacterium, Achromobacter sp. BP3, and cloning of the bph gene cluster
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
علوم زیست محیطی (عمومی)
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چکیده انگلیسی
A bacterial strain, BP3, capable of degrading biphenyl, was isolated from petroleum-contaminated soil. Strain BP3 was identified preliminarily as Achromobacter sp. based on its physiological and biochemical characteristics and 16S rRNA gene sequence analysis. Strain BP3 was able to degrade 50 mg l−1 of biphenyl within 12 h. A 16.7-kb DNA fragment consisting of the entire bph cluster (bphRA1A2XA3A4BCKHJID) was obtained by normal PCR amplification and chromosome walking. Genes encoding integrase and transposon related genes were detected upstream and downstream of the bph cluster, respectively, which indicated that the bph cluster might locate on a big mobile genetic element (MGE).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 63, Issue 4, June 2009, Pages 365–370
Journal: International Biodeterioration & Biodegradation - Volume 63, Issue 4, June 2009, Pages 365–370
نویسندگان
Qing Hong, Xiaojun Dong, Lijuan He, Xin Jiang, Shunpeng Li,