کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4365530 | 1301763 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Crucial factors affecting the nitrile hydratase production of Mesorhizobium sp. F28
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
علوم زیست محیطی (عمومی)
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چکیده انگلیسی
Mesorhizobium sp. F28 contains cobalt-NHase, which effectively converts acrylonitrile into acrylamide. When urea was added to the culture medium, the NHase activity was 62.3Â UÂ mlâ1 (R2A-R2A/urea) after 22.5Â h of cultivation, which was similar to that in the medium without addition (R2A-R2A, 70.0Â UÂ mlâ1). The relative activity of the purified NHase was 100%, 92%, 94%, and 92% in the medium containing, respectively, 0Â mM, 2Â mM, 5Â mM, and 10Â mM of urea. Urea had no significant effect on the purified NHase activity of Mesorhizobium sp. F28. This research did not observe the NHase production by Mesorhizobium sp. F28 when acrylonitrile was supplemented in the culture medium except that cobalt ions existed. The highest enzyme activity was 328.5Â UÂ mlâ1 as cobalt ions were added in the pre-culture and culture medium after 22.5Â h of cultivation (R2A/Co-R2A/Co); compared to media without cobalt ions (R2A-R2A, 22.5Â h, 70.5Â UÂ mlâ1) this is an almost five-fold enhancement. It can be concluded that culture media containing cobalt ions was beneficial for the formation of active NHase of Mesorhizobium sp. F28.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 63, Issue 1, January 2009, Pages 57-61
Journal: International Biodeterioration & Biodegradation - Volume 63, Issue 1, January 2009, Pages 57-61
نویسندگان
Yun-Shu Feng, Chi-Mei Lee,