کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4365972 1301797 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Culturing erosion bacteria: Procedures for obtaining purer cultures and pure strains
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Culturing erosion bacteria: Procedures for obtaining purer cultures and pure strains
چکیده انگلیسی
This paper describes procedures used in attempts to isolate pure strains of erosion bacteria from bacterial consortia obtained from decaying wood. The wood originated from archaeological waterlogged wood and foundation pilings. Kapok fibres were used as a substitute for wood in most experiments. Erosion bacteria attached firmly to the surface of kapok fibres and to cellophane. Intense washing of these substrates resulted in active cultures with a reduced number of associated secondary bacteria. This was not achieved with common dilution techniques. Pure strains obtained from streaking on cellulose or nutrient agar failed to attack kapok fibres and pine wood. Single erosion bacteria cells were separated from secondary bacteria by using laser techniques (optical tweezers). Molecular analyses of the most purified culture, obtained through washing of cellophane, and of laser separated bacteria, suggested that erosion bacteria belong to the CFB (Cytophaga-Flavobacterium-Bacteroides) complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 61, Issue 1, January 2008, Pages 17-23
نویسندگان
, , ,