کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4366129 | 1616545 | 2016 | 12 صفحه PDF | دانلود رایگان |
• The loss of laeA and veA genes led to a drastic reduction of conidia production.
• Deletion of laeA and veA genes strongly comprises mycotoxin production.
• VeA could be linked to CmrA, a regulator of the melanin biosynthesis pathway.
Alternaria alternata is a common filamentous fungus that contaminates various fruits, grains and vegetables causing important economic losses to farmers and the food industry. A. alternata is a mycotoxigenic mould, which may jeopardize human and animal health. Two of the most common A. alternata mycotoxins found in food and feed are alternariol and alternariol monomethyl ether. In this study we examined the role of LaeA and VeA, two regulatory proteins belonging to the velvet family, which have been described to be involved in several functions in many fungi including secondary metabolism. We found that deletion of laeA and veA genes, respectively, greatly reduced sporulation and strongly compromised mycotoxin production, both in vitro or during pathogenesis of tomato fruits. We have also studied how the loss of laeA and veA may affect expression of genes related to alternariol and alternariol monomethyl ether biosynthesis (pksJ and altR), and to melanin biosynthesis (cmrA, pksA).
Journal: International Journal of Food Microbiology - Volume 238, 5 December 2016, Pages 153–164