Bactericidal effect of supercritical N2O on Staphylococcus aureus and Escherichia coli

► We examined the bactericidal activity of SC N2O on both S. aureus and E. coli. ► The bactericidal efficiency was not adversely affected by varying suspending mediums. ► Besides, the present of oleic acid in SC N2O enhanced the bactericidal activity. ► SC N2O technology can be effectively employed as SC CO2 sterilization technique.