Efficiency of different sanitation methods on Listeria monocytogenes biofilms formed under various environmental conditions

► Attachment of L. monocytogenes on SS coupons at 20 °C was higher than at 5 °C. ► Treatment of SS coupons with warm water did not significantly reduce the attached populations. ► Treatment of SS coupons with PAA significantly reduced the levels of attached cells and this reduction increased with exposure time. ► The resistance of attached cells to QAC, PAA and Cl- followed the order: AA-5.0>NA-7.0≥AA-7.0 > NA-5.0. ► The most effective sanitizer was QAC followed by PAA and Cl-, regardless of acid adaptation.