کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4368251 | 1616697 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina Genome analysis of two virulent Streptococcus thermophilus phages isolated in Argentina](/preview/png/4368251.png)
چکیده انگلیسی
Two Streptococcus thermophilus phages (ALQ13.2 and ÏAbc2) were previously isolated from breakdowns of cheese manufacture in Argentina. Complete nucleotide sequence analysis indicated that both phages contained linear double-stranded DNA: 35,525Â bp in length for the pac-type phage ALQ13.2 and 34,882Â bp for the cos-type phage ÏAbc2. Forty-four and 48 open reading frames (ORF) were identified for ALQ13.2 and ÏAbc2, respectively. Comparative genomic analysis showed that these isolates shared many similarities with the eight previously studied cos- and pac-phages infecting different S. thermophilus strains. In particular, part of the ÏAbc2 genome was highly similar to a region of phage 7201, which was thought to be unique to this latter phage. Protein analysis of the pac-phage ALQ13.2 using SDS polyacrylamide gel electrophoresis (SDS-PAGE) identified three major proteins and seven minor proteins. Parallel structural proteome analysis of ÏAbc2 revealed seven protein bands, two of which were related to major structural proteins, as expected for a cos-type phage. Similarities to other S. thermophilus phages suggest that the streptococcal phage diversity is not extensive in worldwide dairy factories possibly because related high-performing bacterial strains are used in starter cultures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 136, Issue 1, 30 November 2009, Pages 101-109
Journal: International Journal of Food Microbiology - Volume 136, Issue 1, 30 November 2009, Pages 101-109
نویسندگان
Daniela M. Guglielmotti, Hélène Deveau, Ana G. Binetti, Jorge A. Reinheimer, Sylvain Moineau, Andrea Quiberoni,