کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4369059 1616736 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Insertion sequence elements as mediators of strain diversity in Lactobacillus helveticus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Insertion sequence elements as mediators of strain diversity in Lactobacillus helveticus
چکیده انگلیسی

Insertion sequence (IS) elements were found to be associated with the truncation of predicted cellobiose transport, acetaldehyde dehydrogenase and diacetyl reductase genes in the genome of Lactobacillus helveticus DPC 4571. The conservation of the IS elements in these different genomic locations among L. helveticus cheese isolates was determined by amplification with gene-specific and IS element-specific primers. The presence of two of the IS elements was found to follow a genotypic profile of the strains generated by randomly amplified polymorphic DNA (RAPD)–PCR and strains that clustered by RAPD–PCR tended to have the IS element in the same position. However, the IS element that interrupted the cellobiose transport gene was found to be common to all strains tested. This conserved genotype suggests the insertion event occurred early in the evolution of L. helveticus as a separate species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 120, Issues 1–2, 30 November 2007, Pages 120–123
نویسندگان
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