کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4370064 1616748 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and identification of Mycobacteria from raw milk samples in Afyonkarahisar district of Turkey
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Isolation and identification of Mycobacteria from raw milk samples in Afyonkarahisar district of Turkey
چکیده انگلیسی

Nontuberculous mycobacteria (NTM) have become a significant cause of infection with the emergence of HIV/AIDS, primarily Mycobacterium avium complex members, and NTM transmitted to humans from the environment (water, fruit, vegetables, beef, and milk). The objectives of this study were to show the presence of mycobacteria in milk and to determine their role as possible sources of human infection. For this, raw milk samples (N = 35) were decontaminated with sodium dodecyl (lauryl) sulfate (SDS)-NaOH and inoculated on to Löwenstein–Jensen medium. After confirming positive 15 slides, isolated from milk by Ziehl–Neelsen technique, they were identified by PCR-Restriction Fragment Length Polymorphism Analysis (PRA). Nine hospital isolates which were identified as Mycobacterium tuberculosis complex by BACTEC were used at control group. We have detected Mycobacterium terrae, Mycobacterium kansassii, Mycobacterium agri at the end of the identification. Two mycobacterial raw milk isolates were not able to be identified by PRA. PRA patterns' were found to be similar to those which obtained University Faculty of Medical by BACTEC. The results showed that raw milk is to be possible sources for human infection. That is why UHF sterilized milk should be consumed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 115, Issue 3, 20 April 2007, Pages 343–347
نویسندگان
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