کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4370977 | 1617002 | 2016 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Expression of Cryptosporidium parvum thioredoxin peroxidase in COS-7 cells confers radioprotection Expression of Cryptosporidium parvum thioredoxin peroxidase in COS-7 cells confers radioprotection](/preview/png/4370977.png)
• CpTPx was transfected to COS-7 cells.
• CpTPx mediated higher cell survival rates among the experimental groups.
• Potent antioxidant and DNA damage protection activity of CpTPx are conserved in cells.
• Apoptotic change was reduced in CpTPx transfected COS-7 after irradiation.
• Transfected CpTPx contribute to radioprotection in COS-7 cells.
Cryptosporidium parvum is one of the most radioresistant organisms identified to date. In a previous study, we found that thioredoxin peroxidase (CpTPx) was significantly upregulated in this species following exposure to high dose (10 kGy) of γ−irradiation. To assess the potential of CpTPx to confer radioprotection in mammalian cells, it was expressed in COS-7 African green monkey kidney cells (CpTPx-COS7). For comparison, the thioredoxin peroxidase of Cryptosporidium muris (CmTPx) was also expressed in these cells (CmTPx-COS7 cells), which has been confirmed to have lesser antioxidant activity than CpTPx in the previous study. Notably, the survival rates of CpTPx-COS7 cells were significantly higher (12–22%) at 72 h after 8 Gy irradiation than CmTPx-COS7 or non-transfected COS-7 (ntCOS-7) counterparts. In addition, CpTPx revealed a 50% of ROS reduction in irradiated CpTPx-COS7 cells, while γ-H2AX DNA damage marker expression was not significantly changed. Furthermore, the amount of apoptosis only increased to about 120% after 2–8 Gy irradiation compared to 200–300% increase observed in ntCOS-7 cells. CmTPx was shown to have antioxidant and DNA damage protection activities; however, these activities were always lower than those of CpTPx. These results suggest that the potent antioxidant and protective activities of CpTPx are well conserved in this cell-based system and that CpTPx contributed to the radioprotection of mammalian cells through its exceptional antioxidant activity.
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Journal: Experimental Parasitology - Volume 163, April 2016, Pages 8–15