کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371133 | 1302474 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The role of Y84 on domain 1 and Y87 on domain 2 of Paragonimus westermani taurocyamine kinase: Insights on the substrate binding mechanism of a trematode phosphagen kinase The role of Y84 on domain 1 and Y87 on domain 2 of Paragonimus westermani taurocyamine kinase: Insights on the substrate binding mechanism of a trematode phosphagen kinase](/preview/png/4371133.png)
• Paragonimus westermani taurocyamine kinase has unique substrate binding mechanism.
• Y84 on D1 and Y87 on D2 of P. westermani TK do not control substrate specificity.
• Residues Y84 and Y87 have significant role in enhancement of taurocyamine binding.
• A59 on D1 and A62 on D2 on the GS region of are also important in substrate binding.
The two-domain taurocyamine kinase (TK) from Paragonimus westermani was suggested to have a unique substrate binding mechanism. We performed site-directed mutagenesis on each domain of this TK and compared the kinetic parameters KmTc and Vmax with that of the wild-type to determine putative amino acids involved in substrate recognition and binding. Replacement of Y84 on domain 1 and Y87 on domain 2 with R resulted in the loss of activity for the substrate taurocyamine. Y84E mutant has a dramatic decrease in affinity and activity for taurocyamine while Y87E has completely lost catalytic activity. Substituting H and I on the said positions also resulted in significant changes in activity. Mutation of the residues A59 on the GS region of domain 1 also caused significant decrease in affinity and activity while mutation on the equivalent position on domain 2 resulted in complete loss of activity.
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Journal: Experimental Parasitology - Volume 135, Issue 4, December 2013, Pages 695–700