In-vitro culture of Plasmodium falciparum: Utility of modified (RPNI) medium for drug-sensitivity studies using SYBR Green I assay

► RPNI, a modified medium can be used for antimalarial chemo-sensitivity studies. ► FBS and ALBUMAX can be used in MSF assay for routine screening of new moieties. ► The fluorescence (MSF) assay is more convenient than radioactive assay.