کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4371352 1302510 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Meloidogyne incognita: Molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Meloidogyne incognita: Molecular cloning and characterization of a cDNA encoding a cathepsin D-like aspartic proteinase
چکیده انگلیسی

Herein we describe the cloning and characterization of a cDNA encoding an aspartic proteinase from the root-knot nematode Meloidogyne incognita. Using PCR techniques, a 1471-bp cDNA fragment encoding a cathepsin D-like (Mi-asp1) transcript was isolated from second-stage larvae mRNA. Its predicted amino acid sequence comprises a pro-region of 71 amino acid residues and a mature protease of 378 amino acid residues with a predicted molecular mass of 41.502 kDa. Protein sequence comparisons of Mi-asp1 with GenBank™ (DQ360827) sequences showed 59–71% identity with nematode-specific cathepsin D-like aspartic proteinases. Southern blot analysis, RT-PCR amplification and EST mining suggest the existence of a developmentally expressed gene family encoding aspartic proteinases in M. incognita. Mi-asp1 may represent a potential target for molecular intervention for the purposes of plant–parasitic nematode control.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 121, Issue 2, February 2009, Pages 115–123
نویسندگان
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