کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371565 | 1302529 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Toxoplasma gondii: Effect of infection on expression of 14-3-3 proteins in human epithelial cells Toxoplasma gondii: Effect of infection on expression of 14-3-3 proteins in human epithelial cells](/preview/png/4371565.png)
14-3-3 Proteins are expressed in most eukaryotes organisms and play varied and crucial roles in a wide range of regulatory processes. In mammalian cells, seven 14-3-3 isoforms have been identified. However, it is not known what effect infection has on 14-3-3 isoform expression. In this study human colonic carcinoma cell lines were infected with Toxoplasma gondii for 24 h and expression of 14-3-3 proteins was determined by RT-PCR. HT-29 cells only expressed 3 out of the 7 isoforms while 5 and all 7 isoforms were found in HCT-116 and Caco-2 cells, respectively. Infection had little or no effect in the expression of 14-3-3γ, ε, σ, and ξ; but in HCT-116 cells induced expression of 14-3-3η and σ, while 14-3-3β, η, and ξ were induced in HT-29 cells. If 14-3-3 proteins are involved in cell survival and/or prevention of parasite replication, longer incubation times may be required as no differences in percentage of infection were found among the cell lines at 24 h post-infection.
Journal: Experimental Parasitology - Volume 118, Issue 1, January 2008, Pages 134–138