کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371688 | 1302535 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Leishmania major: Identification of developmentally regulated proteins in procyclic and metacyclic promastigotes
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کلمات کلیدی
MetacyclicIEFPromastigotes2D-PAGEMOWSE(DTT)peanut agglutinin - آگلوتینین بادام زمینیtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیisoelectric focusing - تمرکز ذره ای الکتریکیdithiothreitol - دیتیوتریتولProcyclic - سالیانهMatrix-assisted laser desorption/ionisation time-of-flight mass spectrometry - طیف سنجی جرمی زمان یخ زدن لیزر جذب / یونیزاسیون ماتریسMALDI-TOF-MS - مالدی TOF-MSProteomics - پروتئومیکس
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The differentiation from procyclic to metacyclic promastigotes (metacyclogenesis) has been correlated with an increased infectivity in a number of Leishmania species. We compared the proteomes of procyclic and metacyclic promastigotes of L. major. Lysates from either life cycle stage were resolved by 2D-PAGE, followed by Coomassie brilliant blue staining. Spots were analyzed by MALDI-TOF MS. 25 protein spots were found to be differentially expressed during metacyclogenesis. We found that proteins involved in protein synthesis were less abundant in metacyclic promastigotes, while proteins involved in motility, including paraflagellar rod protein 1D, α-tubulin and β-tubulin were more abundant. Also, two mitochondrial enzymes (succinyl-CoA synthetase β subunit and cytochrome c oxidase subunit IV) were differentially expressed in both life cycle stages. Down-regulation of proteins related to synthetic pathway in metacyclic promastigotes is consistent with the arrested growth in this life cycle stage, while up-regulation of proteins related to motility in metacyclic promastigotes is in agreement with the high motility observed in this stage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 119, Issue 3, July 2008, Pages 422-429
Journal: Experimental Parasitology - Volume 119, Issue 3, July 2008, Pages 422-429
نویسندگان
Zahra Mojtahedi, Joachim Clos, Eskandar Kamali-Sarvestani,