Babesia rodhaini: The protective effect of pyruvate kinase deficiency in mice

Despite the evidence suggesting that mouse pyruvate kinase (PK) deficiency provides protection against malaria in rodents, there has been no investigation of a parallel protective effect against babesiosis caused by Babesia rodhaini. Here, we examined whether a PK-deficient co-isogenic mouse strain (CBA-Pk-1slc) was protected against B. rodhaini infection. We demonstrated that deficiency in pyruvate kinase correlated with a significant protective effect, with survival rates of 50%, 58% and 56% in groups inoculated with 10, 103 and 105 parasitized erythrocytes, respectively. In contrast, control CBA (CBA-Pk-1+) mice exhibited 100% lethality, regardless of the infectious dose. In addition, CBA-Pk-1slc mice showed decreased levels of parasitemia when compared to CBA-Pk-1+ mice, in groups given 10, 103 or 105 parasitized erythrocytes. These results indicate that similar to PK deficiency in rodents, PK deficiency in mice affects the in vivo growth of B. rodhaini and protects the mice from lethal babesiosis.