کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4371809 1302542 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning and analysis of a new venom allergen-like protein gene from the root-knot nematode Meloidogyne incognita
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Molecular cloning and analysis of a new venom allergen-like protein gene from the root-knot nematode Meloidogyne incognita
چکیده انگلیسی

A new venom allergen-like protein gene isolated from Meloidogyne incognita (designated Mi-vap-2) was cloned and analysed. The genomic clone of Mi-vap-2 is 1917-bp long, contains three introns, which range in size from 39 to 797 bp, and four exons ranging in size from 37 to 361 bp. The cDNA of Mi-vap-2 contains an open reading frame encoding 294 amino acids, being the first 16 residues a putative secretion signal. Southern blot analysis suggested that Mi-vp-2 is probably a member of a small multigene family. In situ hybridization analysis showed that the transcripts of Mi-vap-2 accumulated exclusively within the subventral oesophageal gland cells of M. incognita. RT-PCR analyses confirmed that Mi-vap-2 was transcribed mainly in the pre-parasitic second-stage and early post-inoculated juveniles. Results indicated that this venom allergen-like protein gene may play an important role in establishment of the parasitic relationship between plants and nematodes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 117, Issue 2, October 2007, Pages 133–140
نویسندگان
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