کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4372028 | 1302558 | 2006 | 5 صفحه PDF | دانلود رایگان |
Phagocytosis is the important virulent determinant of the enteric protozoan parasite Entamoeba histolytica. We compared the kinetics of phagosome maturation of attenuated and highly-virulent strains of E. histolytica using video microscopy. Phagosomes of attenuated strains were acidified rapidly within 2 min after phagosome formation (at the rate of 0.96 pH/min), persisted at pH 4.46 ± 0.13, and degraded ingested GFP-Leishmania very efficiently (90–94% GFP fluorescence was lost in 30 min), while phagosomes of highly-virulent strains were acidified slowly (0.69 pH/min), persisted at 5.11 ± 0.23, and degraded GFP less efficiently (60–71% decrease). These results suggest that efficiency of phagosome maturation is most probably inversely correlated with apparent virulence.
Journal: Experimental Parasitology - Volume 114, Issue 1, September 2006, Pages 57–61