کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4372039 | 1302559 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
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![عکس صفحه اول مقاله: Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences](/preview/png/4372039.png)
چکیده انگلیسی
The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene for six Babesia spp. isolated from different geographic origins were characterized. Varying degrees of ITS1 and ITS2 intra- and inter-species sequence polymorphism were found among these isolates. Phylogenetic analysis of the ITS1-5.8S gene-ITS2 region clearly separated the isolates into two clusters. One held an unidentified Babesia sp. transmitted by Hyalomma anatolicum anatolicum. The second held five other isolates, which were considered to be Babesia motasi. Each Babesia species cluster possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. The results showed that ITS1, ITS2 and the complete ITS1-5.8S-ITS2 region could be used to discriminate these ovine Babesia spp. effectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 121, Issue 1, January 2009, Pages 64-68
Journal: Experimental Parasitology - Volume 121, Issue 1, January 2009, Pages 64-68
نویسندگان
Qingli Niu, Jianxun Luo, Guiquan Guan, Zhijie Liu, Miling Ma, Aihong Liu, Jinliang Gao, Qiaoyun Ren, Youquan Li, Jiaxiang Qiu, H. Yin,