The Prediction of Peptide Charge States for Electrospray Ionization in Mass Spectrometry

Electrospray ionization in proteomic mass spectrometry is one of important methods of soft ionization or vaporization for the widest range of polar biomolecules. The number of charges attached onto peptides in ESI can extend the detection limit of mass spectrometer and can be used to estimate the location of mass signal of peptides in the mass spectrum. We present an approach to predict the charge state of peptides according to the composition of peptides, and it has been tested and verified on different datasets. It shows sufficient performance.